In vitro characterization of ligand-induced oligomerization of the S. cerevisiae G-protein Coupled Receptor, Ste2p. Shi, C., Paige, M.F., Maley, J., Loewen, M.C. (2009) Biochimica et Biophysica Acta, 1790:1-7.
This paper represents a collaborative effort between the Loewen Lab at PBI and Dr. Matthew Paige of the Department of Chemistry at the University of Saskatchewan as well as Mr. Jason Maley of the Saskatchewan Structural Sciences Center. Essentially we demonstrate ligand induced oligomerization of Ste2p to dimeric, trimeric and possibly higher order states in detergent solubilized micelles and in isolated cell membranes. Atomic force microscopy, dynamic light scattering and chemical crosslinking methods are applied. Please visit our Publications page to view a PDF version of this paper.