About Us

Summer 2016 - (Left to Right): Anthony Zara, Zerihun Demissie, Elisha Hayer, Melanie Vig, Fang Huang & Michele Loewen.
Currently we are investigating mechanistic aspects of fungal disease resistance, with a focus on transport specificities and associated mechanisms of action for ABC transporters (and a few enzymes). We rely on recombinantly produced and enriched proteins, applied to enzyme kinetic studies and proteoliposome transport assays to gain knowledge about the target protein's transported allocrites, enzymatic products and mechanisms of action. We also do some industrial protein engineering.

New Publication – Shi et al (2009)

Dec 2nd, 2008 by admin | 0

In vitro characterization of ligand-induced oligomerization of the S. cerevisiae G-protein Coupled Receptor, Ste2p. Shi, C., Paige, M.F., Maley, J., Loewen, M.C. (2009) Biochimica et Biophysica Acta, 1790:1-7.

This paper represents a collaborative effort between the Loewen Lab at PBI and Dr. Matthew Paige of the Department of Chemistry at the University of Saskatchewan as well as Mr. Jason Maley of the Saskatchewan Structural Sciences Center. Essentially we demonstrate ligand induced oligomerization of Ste2p to dimeric, trimeric and possibly higher order states in detergent solubilized micelles and in isolated cell membranes. Atomic force microscopy, dynamic light scattering and chemical crosslinking methods are applied. Please visit our Publications page to view a PDF version of this paper.

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