{"id":876,"date":"2022-02-01T09:28:47","date_gmt":"2022-02-01T15:28:47","guid":{"rendered":"http:\/\/www.loewenlabs.com\/michele\/wordpress\/?page_id=876"},"modified":"2022-10-01T07:16:18","modified_gmt":"2022-10-01T13:16:18","slug":"lab-overview","status":"publish","type":"page","link":"https:\/\/www.loewenlabs.com\/michele\/wordpress\/","title":{"rendered":"Lab Overview"},"content":{"rendered":"<h1><strong>Research<\/strong><\/h1>\n<ul>\n<li>Industrial Enzyme Engineering<\/li>\n<li>G-protein coupled receptors (GPCRs) in infection and disease.<\/li>\n<li>Mechanism of fungal biocontrol<\/li>\n<\/ul>\n<p>On the one hand we are engaged in industrial enzyme engineering toward the development of novel bioprocessing technologies to apply to industrial waste streams for the production of value added products in the mining, forestry, textiles and feed\/food industries.<\/p>\n<p class=\"x_MsoNormal\">On the other hand our lab is investigating mechanistic aspects of fungal pathogenesis and related fungal biocontrol. This includes a particular focus on <i>Fusarium graminearum<\/i>, a devastating fungal pathogen of cereal crops that both decreases grain quality and yield as well as deposits mycotoxins. <i>Fusarium<\/i> has become significantly more prevalent in Canada recently, likely related to climate change. Toward addressing <i>Fusarium<\/i>, we are also studying the mechanisms underlying the fungal biocontrol agent <i>Clonostachy rosea<\/i>, shown to have growth inhibition activity toward <i>Fusarium<\/i>. We use genetic, transcriptomic, metabolomic, biochemical and biophysical methods to probe fungal perception and signal transduction as well as metabolic pathways underlying the pathogenic and biocontrol functionalities of these fungal species. Assays use a variety of different analytical methods such as liquid chromatography, mass spectroscopy, photospectroscopy and nuclear magnetic resonance for metabolite analyses. We also use protein crystallography to better understand protein-structure function relationships.<\/p>\n<h1 class=\"x_MsoNormal\"><strong>Publications, Protein Structures and Patents<br \/>\n<\/strong><\/h1>\n<p>Our 3 latest publications include:<\/p>\n<div class=\"cit\">\n<p class=\"title hypothesis_container\">Mohapatra DP, Robinson KA, Huang F, Kirpalani\u00a0 D, Loewen MC (<strong>2022<\/strong>) Insights into Increasing Selenate Reductase Enzyme Activity in the Presence of Nitrogen-Doped Graphite Electrodes for Selenium Effluent Treatment. <strong>Water<\/strong> 14:931. [<a href=\"https:\/\/www.mdpi.com\/2073-4441\/14\/6\/931\/htm\">online<\/a>]<\/p>\n<p>Buhrow LM, Liu Z, Cram D, Sharma T, Foroud NA, Pan Y, Loewen MC. (<strong>2021<\/strong>)Wheat transcriptome profiling reveals abscisic and gibberellic acid treatments regulate early-stage phytohormone defense signaling, cell wall fortification, and metabolic switches following Fusarium graminearum-challenge.<strong>BMC Genomics<\/strong> 22:798. [<a href=\"https:\/\/bmcgenomics.biomedcentral.com\/articles\/10.1186\/s12864-021-08069-0 target=\">online<\/a>]<\/p>\n<p>Demissie ZA, Robinson KA, Loewen MC (<strong>2021<\/strong>) Draft genome resources for the plant-beneficial fungi <em>Clonostachys rosea<\/em> strains ACM941 and 88-710. <strong>Molecular Plant-Microbe Interactions<\/strong>, doi: 10.1094\/MPMI-10-20-0294-A. [<a href=\"https:\/\/apsjournals.apsnet.org\/doi\/pdf\/10.1094\/MPMI-10-20-0294-A\">online<\/a>]<\/p>\n<div class=\"cit\">\n<p class=\"desc\">Please see our full list of publications in <strong><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=loewen+mc&amp;sort=date\" target=\"_blank\" rel=\"noopener noreferrer\">PubMed<\/a><\/strong><br \/>\nPlease also enjoy our 6 most recent\u00a0 protein structures listed in the\u00a0 RCSB<strong><strong><strong>: <a href=\"https:\/\/www.rcsb.org\/structure\/7U1H\">7U1H<\/a>, <a href=\"https:\/\/www.rcsb.org\/structure\/7U1I\">7U1I<\/a>, <a href=\"https:\/\/www.rcsb.org\/structure\/7U1J\">7U1J<\/a>, <a href=\"http:\/\/www.rcsb.org\/structure\/5V2D\">5V2D,<\/a> <a href=\"http:\/\/www.rcsb.org\/structure\/4MKV\">4MKV<\/a>, <a href=\"http:\/\/www.rcsb.org\/structure\/4HHH\">4HHH<\/a>.<\/strong><\/strong><\/strong><br \/>\nPlease also see our patent at <strong><strong><a href=\"http:\/\/patft.uspto.gov\/netacgi\/nph-Parser?Sect1=PTO2&amp;Sect2=HITOFF&amp;p=1&amp;u=%2Fnetahtml%2FPTO%2Fsearch-bool.html&amp;r=2&amp;f=G&amp;l=50&amp;co1=AND&amp;d=PTXT&amp;s1=8,283,481&amp;OS=8,283,481&amp;RS=8,283,481\" target=\"_blank\" rel=\"noopener noreferrer\">USPTO<\/a><\/strong><\/strong><strong>\u00a0<\/strong><\/p>\n<h1><strong>Current Group Members<\/strong><\/h1>\n<p class=\"x_MsoNormal\"><strong>Michele C. Loewen<\/strong><br \/>\nSenior Research Officer<br \/>\nAdjunct Professor &#8211; <a href=\"https:\/\/dbms.queensu.ca\/faculty\/michele-loewen\" target=\"_blank\" rel=\"noopener noreferrer\">QueensU<\/a><br \/>\nAdjunct Professor &#8211; <a href=\"https:\/\/science.uottawa.ca\/chemistry\/people\/loewen-michele\" target=\"_blank\" rel=\"noopener noreferrer\">uOttawa<\/a><\/p>\n<p><strong>Pooja Sridhar<\/strong> (Ph.D. Student; QueensU)<br \/>\n<strong>Kelly Robinson<\/strong> (Technical Officer; NRC)<br \/>\n<strong>Tanya Sharma<\/strong> (Ph.D. Student; uOttawa)<br \/>\n<strong>Joshua Lister<\/strong> (M.Sc. Student; uOttawa)<br \/>\n<strong>Peter Adewale<\/strong> (Research Associate; NRC)<br \/>\n<strong>Antony St-Jacques<\/strong> (Research Associate; NRC)<br \/>\n<strong>Deandre Chevannes<\/strong> (M.Sc. Student; uOttawa)<br \/>\n<strong>Joanna<\/strong> <strong>Stanczyk<\/strong> (Coop Student; QueensU)<br \/>\n<strong>Yutong Zhang<\/strong> (M.Sc. Student; uOttawa)<br \/>\n<strong>Idin Lantz<\/strong> (Undergraduate Project Student; uOttawa)<br \/>\n<strong>Luana Porto<\/strong> (Post-Doctoral Associate; uOttawa)<\/p>\n<p>Please see also our lab <strong><a href=\"https:\/\/www.loewenlabs.com\/michele\/wordpress\/alumni\/\" target=\"_blank\" rel=\"noopener noreferrer\">Alumni<\/a><\/strong><\/p>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>Research Industrial Enzyme Engineering G-protein coupled receptors (GPCRs) in infection and disease. Mechanism of fungal biocontrol On the one hand we are engaged in industrial enzyme engineering toward the development of novel bioprocessing technologies to apply to industrial waste streams for the production of value added products in the mining, forestry, textiles and feed\/food industries. &hellip; <\/p>\n<p class=\"link-more\"><a href=\"https:\/\/www.loewenlabs.com\/michele\/wordpress\/\" class=\"more-link\">Continue reading<span class=\"screen-reader-text\"> &#8220;Lab Overview&#8221;<\/span><\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-876","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/www.loewenlabs.com\/michele\/wordpress\/wp-json\/wp\/v2\/pages\/876","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.loewenlabs.com\/michele\/wordpress\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.loewenlabs.com\/michele\/wordpress\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.loewenlabs.com\/michele\/wordpress\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.loewenlabs.com\/michele\/wordpress\/wp-json\/wp\/v2\/comments?post=876"}],"version-history":[{"count":66,"href":"https:\/\/www.loewenlabs.com\/michele\/wordpress\/wp-json\/wp\/v2\/pages\/876\/revisions"}],"predecessor-version":[{"id":1093,"href":"https:\/\/www.loewenlabs.com\/michele\/wordpress\/wp-json\/wp\/v2\/pages\/876\/revisions\/1093"}],"wp:attachment":[{"href":"https:\/\/www.loewenlabs.com\/michele\/wordpress\/wp-json\/wp\/v2\/media?parent=876"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}